Dot is of the same species as her older brothers. She wears a pink skirt, pink or frilly white panties, has hair resembling that of Uran from Astro Boy, and a yellow flower tie in her ears. In Wakko's Wish, she gets a beauty mark that she calls a "cutie mark" ("Cindy Crawford, eat your heart out!" she remarked). However, this "cutie mark" only appeared in the movie. Whenever Yakko and Wakko introduce themselves by saying "We're the Warner Brothers!", she always follows with "And the Warner Sister!" Her full name is "Princess Angelina Contessa Louisa Francesca Banana Banna Bo Besca the Third" (a reference to Pippi Longstocking's long real name, "Pippilotta Delicatessa Windowshade Mackrelmint Ephraim's Daughter Longstocking"), which is the reason why some fans call her "Princess Dot" for short, as revealed in Wakko's Wish. (Her last name is Warner, but is not added to her full name.) According to Tom Ruegger, Dot is 9 years old.
Extremely cute, beautiful and thoroughly aware of it, Dot is very confident, but a bit vain and selfish at times. She is arguably the cutest one; she even sings a whole song about how cute she is in the episode "I'm Cute". She is also the most overtly aggressive. As she says in the first episode, "Call me Dottie, and you die." However, she's been called Dottie a few times and the source remains unharmed, but this is not always true. In one episode, she throws a bomb at Yakko and Wakko for calling her that. She often makes note of Wakko's ridiculous and disgusting eating habits (even saying he went too far when he ate the moon in one episode), going "DIIIIIISGUSTING!" whenever he eats something unappetizing. She is easily miffed at her brothers' reactions around females; afterwards she often says, "Boys: go fig." She loves her brothers dearly, but their behavior (notably around women) often annoys her.
However, even though she views Yakko and Wakko's behavior around beautiful women repugnant, she has reacted similarly to attractive men, most notably Mel Gibson (She said she would "marry that man"). Dot also carries around a pet, contained within a tiny box that is revealed to be a horrifying monster much too large to have fit inside the box. In one episode, one of her pets ends up marrying a Xenomorph. She also seems to have a sense of humor much like her brother Yakko. But, like Wakko and his hat, even when Dot is in a costume she usually has a little flower in her ears or hat (unless she is in her pajamas and/or going to sleep, then she wears curlers). Her voice is like that of Babs Bunny (also voiced by MacNeille) and later Fang on Dave the Barbarian, but with a younger, sweeter tone to match with her apparent age as the youngest sibling. Also, as explained in the Wakko's Wish, it has been discovered that Dot was born from a beautiful flower in a garden on the first day of spring. However, when Yakko tells her this story at bedtime, it sounds fictional.